vectorbee
About VectorBee
VectorBee is a highly user-friendly genetic engineering software created by VectorBuilder for viewing, editing and analyzing DNA, RNA, and protein sequences. It is developed by a talented team of biologists and IT engineers led by Professor Bruce Lahn. As the Chief Scientist of VectorBee (and also VectorBuilder), Bruce has decades of know-how in molecular biology with over 100 publications, so he knows intuitively what functionalities his peers need in VectorBee. Importantly, Bruce is committed to supporting the research community by making VectorBee 100% free for all and forever.
Since its launch in 2024, VectorBee has amassed hundreds of thousands of users and is quickly becoming one of the most popular software in life sciences, earning glowing testimonials from countless users. Meanwhile, we are working tirelessly to add new features while improving existing ones to meet growing user demands.
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If you need any assistance, please contact us at vectorbeesupport@vectorbuilder.net.
Getting Started with VectorBee
Getting Started with VectorBee
How to Use Vector Design Wizard
How to Use Vector Design Wizard
Comprehensive VectorBee Tutorial
Comprehensive VectorBee Tutorial
About VectorBuilder
VectorBuilder, the creator of VectorBee, is a global leader in gene delivery technologies that offers a wide spectrum of gene delivery solutions covering all research and clinical needs, including vector design, plasmid cloning, virus packaging, cell line engineering, directed evolution, GMP manufacturing, and many more. VectorBee is the latest addition to this growing portfolio. Having serviced tens of thousands of labs and biotech/pharma companies across the globe, VectorBuilder's goal is to make research easy and fun. Give VectorBee a try and get amazed by how this little bee can empower your research!
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Download VectorBee 100% Free for Everyone, Forever! See our free pledge
If your organization blocks the installation of third-party software, please contact us at vectorbeesupport@vectorbuilder.net. We will communicate with your organization to include our software on their approved list.
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If your organization blocks the installation of third-party software, please contact us at vectorbeesupport@vectorbuilder.net. We will communicate with your organization to include our software on their approved list.
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Mac
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Linux
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If your organization blocks the installation of third-party software, please contact us at vectorbeesupport@vectorbuilder.net. We will communicate with your organization to include our software on their approved list.
What's New in VectorBee 3.5.0?
VectorBee 3.5.0 is now available, featuring 6 new and optimized features, including:
These updates enable you to perform more diverse research tasks directly within the software. Below is the full list of VectorBee 3.5.0 updates:
Click here for full list of updates.
Highlights of VectorBee
Fast and effective design with the Vector Design Wizard
Custom experiment-ready vectors designed with just a few clicks and delivered quickly to your bench
Diverse collection of vector backbones and a comprehensive database of vector components
Seamless integration with VectorBuilder
Integration of vectors, backbones, and components from VectorBuilder’s databases; expert technical support; expansive tools; and easy ordering
Quickly submit your vector designs to VectorBuilder to order services such as cloning, plasmid prep, virus packaging, library construction, stable cell line engineering, and more
Easy sequence viewing and editing
Unlimited customization for both viewing and editing sequences
Intuitive interface for visualizing sequences, features, restriction enzyme cut sites, primer designs, and translations
Unlimited sequence annotation
Flexible annotation of features, primers, and restriction enzyme cut sites
Built-in feature library for fast, accurate identification of common sequence features
Nearly 700 enzymes to view and simulate restriction digestion of your sequence
Multi-source sequence import
Import common file formats, including GenBank, FASTA, SnapGene, and more
Import genes directly from NCBI, including genomic, CDS, or transcript sequences, with annotation
Pairwise or multiple sequence alignment
Rich alignment tools, including pairwise and multiple sequence alignment and pairwise sequence dot plot, for assessment of sequence similarity and visualization of complex sequence structures
Realistic agarose gel simulation
Highly realistic and adaptable simulated gel electrophoresis for visualizing restriction digestion results of your vector
Customizable, high-fidelity primer design
Powerful and user-friendly primer design tool for a wide range of applications
Flexibility with multi-primer import and search function to find primer binding sites
Global sequence view with GC content and CpG islands
Detailed maps of GC content and CpG/GpC frequency, along with automatic detection of CpG islands
Customizable project organization
Project management and organization with the Project Manager, including editing descriptions, comments, and binders
Extensive collection of Bio Tools
Comprehensive biological tools to complete more research tasks all in one place
Testimonials
beeIcon Dr. Charles Xu The Rockefeller University, USA
VectorBee is an amazing software for molecular cloning designs and sequence alignments. It is the best alternative to other commercial software. And it is offered free of charge. Thank you so much for providing such great service to the research community!
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beeIcon Pierre-Marie Girard Institut Curie, France
I received a couple of weeks ago two plasmids to express transcripts in vitro, which then are electroporated into mammalian cells to be translated. I had little information about them, apart from having access to the full sequences, and the position of the genes of interest to be transcribed. My idea was to subclone these genes into a mammalian expression vector in order to transfect them into mammalian cells and to have the gene transcribed and translated in cellulo. I first tried online free software but failed to manipulate my sequence easily. Then I used VectorBee and, not only it is a friendly interface easy to use, but in few minutes I managed to create my vectors of interest, with selected promoter, resistance gene, and so on. Amazing software, very easy to use.
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beeIcon Dr. Ekaterina Boyarchuk Centre National de la Recherche Scientifique (CNRS), France
Congratulations to your company on creating such excellent software. It's a real timesaver!
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beeIcon Chun Hao Wong University of Cambridge, UK
As a first-time user, I was impressed by how accessible and user-friendly VectorBee's software is. The ability to feed designs directly to production on VectorBuilder is a standout feature that really streamlines the workflow. The team was incredibly responsive and knowledgeable. Being able to iterate on designs with the team quickly within the software is super valuable.
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beeIcon Dr. Fraser Johnson Netherlands Cancer Institute, Netherlands
I really like this software. It's a great free alternative to paid software.
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beeIcon Dr. Dario Besusso University of Milan, Italy
This tool is getting awesome! It reproduces most of the features that can be found in proprietary software and it works seamlessly with VectorBuilder services allowing straightforward design of complex molecular structures leveraging off-the-shelf plasmids available in their database.
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beeIcon Tobin Xiang Chinese Institute for Brain Research, China
Thank you for your great software VectorBee. In these days, I have used VectorBee as an alternative to commercial software which meets most of the functions I need.
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beeIcon Dr. Ryoji Fukabori Fukushima Medical University, Japan
Japan VectorBee is the best app I have used. My favorite feature is the "Insert Component" function, which significantly reduces the time I spend manually searching for and copying sequences from GenBank or other sequence databases. I also appreciate the "Detect Common Features" function, which automatically adds annotations to my older sequence files after converting them to the VectorBee format. I love these :)
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beeIcon Anne Macy University of Arizona College of Medicine - Phoenix, USA
These are the nicest looking maps I've seen, and I'd like to include them in my publication (citing VectorBee, of course).
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beeIcon Angelique Krempler Georg-August-Universität Göttingen, Germany
First of all, I adore your program, it is really nice to work with.
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beeIcon Dr. Ozdemirhan Sercin German Aerospace Center (DLR)
All in all, it completely replaced commercial software without any major downsides. I am very happy about it, my group and I enjoy using it on a daily basis. Snapgene pricing is a crime. We just want to do science, it feels like a crime to make it easy without paying for massive licence fees.
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beeIcon Maria Ingaramo NonFiction Laboratories Inc., USA
I'm absolutely loving VectorBee. It is super user-friendly and has made designing constructs even more fun. Plus, the team behind it is incredibly responsive, constantly adding new custom features. Thank you for all the great work and dedication that goes into it. I can't wait to see what comes next!
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beeIcon Umang Goswami Abilita Therapeutics, USA
Kudos to the team for developing a great product. Thank you for making the software and having it available for use.
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beeIcon Dr. Jakkrit Amornvit Chulalongkorn University
I really enjoy using your application, the interface is very user-friendly and convenient.
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beeIcon Krisztina Szilagyi Gedeon Richter Pharma GmbH, Germany
Thank you for the opportunity to use this great software to visualize and easily plan everyday DNA work. What I appreciate most about VectorBee is how well it meets a wide range of research needs, making my daily work much easier and more efficient. I would also like to highlight the fantastic support team behind the software - they are always quick, helpful, and professional. The continuous improvements and updates are also a great advantage, as they consistently make the software even better and more user-focused. All of this is outstanding on its own, but what truly makes VectorBee exceptional - and most useful - is when it's used together with the VectorBuilder plasmid design platform. The plasmids designed there can be easily reviewed, analyzed, and studied later in VectorBee in a very user-friendly way.
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beeIcon Susanne Mellert University of Bayreuth, Germany
Thank you very much for prkviding such great software. I love using VectorBee at work. It's so much more intuitive to use than commercial alternatives. Also, the visualisation of alignments is so user-friendly.
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beeIcon Dr. Zubair Khalid University of Maryland, USA
I've been truly impressed by the outstanding work behind Vector Bee. The software offers an exceptionally intuitive and research-friendly interface that makes molecular biology workflows seamless. I particularly appreciate the side-by-side plasmid and sequence views, which make editing and annotation so much more efficient. The alignment tools with bidirectional ORF visualization are another fantastic feature, they provide clarity and precision that’s invaluable for both basic and applied research. Compared to a paid alternative program, it is much smoother and faster on my MacBook. The project-based organization is also a huge advantage, keeping complex experiments well-structured and easy to revisit. In short, Vector Bee become an indispensable tool for me for my day-to-day tasks in the lab.
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beeIcon Yonika Larasati University of Geneva, Switzerland
I would like to thank you all for a great job in developing VectorBee. I've been using it in the past year and it quickly became a crucial tool in my cloning experiment.
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VectorBee 3.5.0 was released on July 7, 2026, including 6 new and optimized features. Click here for details.